Getting Started with Instruments
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Before you begin working with Instruments, ensure you understand the prerequisites and basic setup requirements. This guide will walk you through the initial steps of setting up and using Instruments in your environment.
Before using Instruments, you will need:
A account with appropriate permissions.
Access to cloud storage containing your sequencing data (, , or ).
The and configured.
Use the native tools provided by your cloud service provider to upload data to their storage services. This ensures compatibility and ease of use.
For AWS, use the or the S3 Console.
For Azure, use the or the Azure Portal.
For Google Cloud, use the or the Cloud Storage Console.
Maintain the directory structure and file naming conventions for accurate indexing.
Metadata File and Directory Structure
Workbench relies on the metadata.xml files generated by SMRT Link to identify samples and their associated Movie BAM files.
These metadata files map each sample to the correct data files, enabling Workbench to automatically associate the appropriate files.
It is critical to preserve the directory structure and metadata files as generated by PacBio instruments during upload to ensure seamless detection.
Using SMRT Link to Sync Data to the Cloud
SMRT Link version 25.1 and later supports native cloud storage synchronization.
This feature allows users to directly sync sequencing data, including metadata.xml and BAM files, to a cloud storage bucket of their choice.
Workbench Instruments supports indexing and analyzing the results of PacBio sequencing runs from the on the Revio and Vega platforms. All output data is organized into a directory structure reflecting the SMRT Cells that were run. Barcoding within a SMRT Cell delineates different samples, while unbarcoded Movie BAM files indicate a single sample for the entire SMRT Cell.